Only a few diagnostic tests are commercially available for Ebola disease and these are specific to Ebola virus disease. According to Directive 2000/54/EC of the European Parliament and of the Council, the ebolaviruses are group 4 biological agents [1]. Therefore, samples from infected patients should be handled under strict biological containment conditions in biosafety level 3 (e.g. RT-PCR and enzyme-linked immunosorbent assay on non-inactivated samples) or level 4 laboratories (virus isolation). Any attempt at viral replication should be handled in biosafety level 4 laboratories [19] [20]. For inactivated samples, RT-PCR and ELISA testing can be performed at a laboratory with BSL-2 facilities.
Level 4: Virus Hunters of the CDC - Tracking Ebola and the World's Deadliest Viruses download
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